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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CST4 ORF mammalian expression plasmid, C-GFPSpark tag||HG11542-ACG|
|Human CST4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11542-ACR|
|Human CST4 ORF mammalian expression plasmid, C-Flag tag||HG11542-CF|
|Human CST4 ORF mammalian expression plasmid, C-His tag||HG11542-CH|
|Human CST4 ORF mammalian expression plasmid, C-Myc tag||HG11542-CM|
|Human CST4 ORF mammalian expression plasmid, C-HA tag||HG11542-CY|
|Human CST4 natural ORF mammalian expression plasmid||HG11542-M-N|
|Human CST4 ORF mammalian expression plasmid, N-Flag tag||HG11542-NF|
|Human CST4 ORF mammalian expression plasmid, N-His tag||HG11542-NH|
|Human CST4 ORF mammalian expression plasmid, N-Myc tag||HG11542-NM|
|Human CST4 ORF mammalian expression plasmid, N-HA tag||HG11542-NY|
|Human CST4 natural ORF mammalian expression plasmid||HG11542-UT|
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Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to the cystatin family. Cystatin-4 / CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4 / CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4 / CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.