|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FCRL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11536-ACG|
|Human FCRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11536-ACR|
|Human FCRL1 ORF mammalian expression plasmid, C-Flag tag||HG11536-CF|
|Human FCRL1 ORF mammalian expression plasmid, C-His tag||HG11536-CH|
|Human FCRL1 ORF mammalian expression plasmid, C-Myc tag||HG11536-CM|
|Human FCRL1 ORF mammalian expression plasmid, C-HA tag||HG11536-CY|
|Human FCRL1 Gene cDNA clone plasmid||HG11536-M|
|Human FCRL1 ORF mammalian expression plasmid, N-Flag tag||HG11536-NF|
|Human FCRL1 ORF mammalian expression plasmid, N-His tag||HG11536-NH|
|Human FCRL1 ORF mammalian expression plasmid, N-Myc tag||HG11536-NM|
|Human FCRL1 ORF mammalian expression plasmid, N-HA tag||HG11536-NY|
|Human FCRL1 natural ORF mammalian expression plasmid||HG11536-UT|
|Learn more about expression Vectors|
Fc receptor-like protein 1, also known as FcR-like protein 1, Fc receptor homolog 1, IFGP family protein 1, Immune receptor translocation-associated protein 5 and FCRL1, is a single-pass type I membrane protein which contains three Ig-like C2-type (immunoglobulin-like) domains. It is a cell-surface membrane protein belonging to FCRL family and is preferentially expressed on B cells. FCRL1 is primarily expressed in secondary lymphoid tissues by mature subsets of B cells. It is detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. FCRL1 is specifically expressed by mature B lineage cells with higher expression in naive versus memory B cells (at protein level). Human Fc receptor-like molecules (FCRL1, FCRL2, FCRL3, FCRL4, FCRL5) have tyrosine-based immunoregulatory potential and are expressed by B-lineage subpopulations. FCRL1 may function as an activating coreceptor in B cells. It may also function in B cells activation and differentiation.