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Human MTOR natural ORF mammalian expression plasmid

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Human MTOR cDNA Clone Product Information
NCBI RefSeq:NM_004958.3
RefSeq ORF Size:4049bp
cDNA Description:Full length Clone DNA of Homo sapiens mechanistic target of rapamycin (serine/threonine kinase).
Gene Synonym:FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FLJ44809, MTOR
Species:Human
Vector:pCMV/hygro
Plasmid:pCMV-MTOR(H+P)
Restriction Site:HindIII + PmeI (destroyed)
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 4731 G/A not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human MTOR Gene Plasmid Map
Human MTOR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
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