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Human UBE2D1 Gene cDNA Clone (full-length ORF Clone)

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UBE2D1/UBCH5cDNA Clone Product Information
Gene_bank_ref_id:NM_003338.3
cDNA Size:444
cDNA Description:ORF Clone of Homo sapiens ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast) DNA.
Gene Synonym:SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, UBE2D1
Species:Human
Vector:pMD18-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Vector Information

pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which multiple cloning sites as shown below. The pMD18-T Vector is 2.6kb in size and contains the amplicin resistance gene for selection. The coding sequence was inserted by TA cloning at site 425.

pMD18-T vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

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References
  • Zhang L, et al. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74.
  • Tokumoto M, et al. (2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200.
  • Ohbayashi N, et al. (2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12.
  • Gehrke SG, et al. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.
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    Catalog:HG11432-M
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