|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PTMA ORF mammalian expression plasmid, C-GFPSpark tag||HG11407-ACG|
|Human PTMA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11407-ACR|
|Human PTMA ORF mammalian expression plasmid, N-GFPSpark tag||HG11407-ANG|
|Human PTMA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11407-ANR|
|Human PTMA ORF mammalian expression plasmid, C-Flag tag||HG11407-CF|
|Human PTMA ORF mammalian expression plasmid, C-His tag||HG11407-CH|
|Human PTMA ORF mammalian expression plasmid, C-Myc tag||HG11407-CM|
|Human PTMA ORF mammalian expression plasmid, C-HA tag||HG11407-CY|
|Human PTMA ORF mammalian expression plasmid, N-Flag tag||HG11407-NF|
|Human PTMA ORF mammalian expression plasmid, N-His tag||HG11407-NH|
|Human PTMA ORF mammalian expression plasmid, N-Myc tag||HG11407-NM|
|Human PTMA ORF mammalian expression plasmid, N-HA tag||HG11407-NY|
|Human PTMA natural ORF mammalian expression plasmid||HG11407-UT|
|Learn more about expression Vectors|
PTMA (prothymosin, alpha, N-GST chimera) is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin a1. Thymosins are named becaues they were originally isolated from the thymus. But now in many other tissues, thymosins also can be detected. Thymosins have diverse biological activities, and two in particular, thymosins a1 and _4, have potentially important uses in medicine, some of which have already progressed from the laboratory to the clinic. In general, PTMA is associated with cellular proliferation and carcinogenesis (Eschenfeldt et al., 1986), cellular and viral transcription (Cotter et al., 2000), protection against apoptosis and chromatin remodelling (Karetsou et al., 1998). PTMA may have a dual role both intracellulary and extracellulary. In relation to diseases, thymosins have been categorized as biological response modifiers. Thymosin a1 is derived from PTMA. For animals that lack thymus glands, thymosin a1 is responsible for the activity of that preparation in restoring immune function.