|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CMBL ORF mammalian expression plasmid, C-GFPSpark tag||HG11404-ACG|
|Human CMBL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11404-ACR|
|Human CMBL ORF mammalian expression plasmid, N-GFPSpark tag||HG11404-ANG|
|Human CMBL ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11404-ANR|
|Human CMBL ORF mammalian expression plasmid, C-Flag tag||HG11404-CF|
|Human CMBL ORF mammalian expression plasmid, C-His tag||HG11404-CH|
|Human CMBL ORF mammalian expression plasmid, C-Myc tag||HG11404-CM|
|Human CMBL ORF mammalian expression plasmid, C-HA tag||HG11404-CY|
|Human CMBL Gene cDNA clone plasmid||HG11404-M|
|Human CMBL ORF mammalian expression plasmid, N-Flag tag||HG11404-NF|
|Human CMBL ORF mammalian expression plasmid, N-His tag||HG11404-NH|
|Human CMBL ORF mammalian expression plasmid, N-Myc tag||HG11404-NM|
|Human CMBL ORF mammalian expression plasmid, N-HA tag||HG11404-NY|
|Human CMBL natural ORF mammalian expression plasmid||HG11404-UT|
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Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.