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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human MDGA2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11372-ACG|
|Human MDGA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11372-ACR|
|Human MDGA2 ORF mammalian expression plasmid, C-Flag tag||HG11372-CF|
|Human MDGA2 ORF mammalian expression plasmid, C-His tag||HG11372-CH|
|Human MDGA2 ORF mammalian expression plasmid, C-Myc tag||HG11372-CM|
|Human MDGA2 ORF mammalian expression plasmid, C-HA tag||HG11372-CY|
|Human MDGA2 ORF mammalian expression plasmid, N-Flag tag||HG11372-NF|
|Human MDGA2 ORF mammalian expression plasmid, N-His tag||HG11372-NH|
|Human MDGA2 ORF mammalian expression plasmid, N-Myc tag||HG11372-NM|
|Human MDGA2 ORF mammalian expression plasmid, N-HA tag||HG11372-NY|
|Human MDGA2 natural ORF mammalian expression plasmid||HG11372-UT|
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Mouse MAM domain-containing glycosylphosphatidylinositol anchor protein 2, also known as MAM domain-containing protein 1, MDGA2 and MAMDC1, is a cell membrane protein which contains six Ig-like (immunoglobulin-like) domains and one MAM domain. Analyses of the full-length coding region of MDGA1 and MDGA2 indicate that they encode proteins that comprise a novel subgroup of the Ig superfamily and have a unique structural organization consisting of six immunoglobulin (Ig)-like domains followed by a single MAM domain. Biochemical characterization demonstrates that MDGA1 and MDGA2 proteins are highly glycosylated, and that MDGA1 is tethered to the cell membrane by a GPI anchor. The MDGAs are differentially expressed by subpopulations of neurons in both the central and peripheral nervous systems, including neurons of the basilar pons, inferior olive, cerebellum, cerebral cortex, olfactory bulb, spinal cord, and dorsal root and trigeminal ganglia. The similarity of MDGAs to other Ig-containing molecules and their temporal-spatial patterns of expression within restricted neuronal populations, for example migrating pontine neurons and D1 spinal interneurons, suggest a role for these novel proteins in regulating neuronal migration, as well as other aspects of neural development, including axon guidance.