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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CDH17 ORF mammalian expression plasmid, C-GFPSpark tag||HG11360-ACG|
|Human CDH17 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11360-ACR|
|Human CDH17 ORF mammalian expression plasmid, C-Flag tag||HG11360-CF|
|Human CDH17 ORF mammalian expression plasmid, C-His tag||HG11360-CH|
|Human CDH17 ORF mammalian expression plasmid, C-Myc tag||HG11360-CM|
|Human CDH17 ORF mammalian expression plasmid, C-HA tag||HG11360-CY|
|Human CDH17 natural ORF mammalian expression plasmid||HG11360-M-N|
|Human CDH17 ORF mammalian expression plasmid, N-Flag tag||HG11360-NF|
|Human CDH17 ORF mammalian expression plasmid, N-His tag||HG11360-NH|
|Human CDH17 ORF mammalian expression plasmid, N-Myc tag||HG11360-NM|
|Human CDH17 ORF mammalian expression plasmid, N-HA tag||HG11360-NY|
|Human CDH17 natural ORF mammalian expression plasmid||HG11360-UT|
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Cadherin-17 or LI-cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Cadherin-17/LI-cadherin is a cadherin-like protein consisting of an extracellular region, 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing of the encoding gene results in multiple transcript variants. Cadherin-17/LI-cadherin preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-17 may thus contribute to the sorting of heterogeneous cell types and have a role in the morphological organization of liver and intestine. It's also involved in intestinal peptide transport. Experiments have reported the association between Cadherin-17/LI-cadherin and gastric cancer. Cadherin-17/LI-cadherin expression was detected in 63/94 of gastric adenocarcinomas in addition to intestinal metaplasia. The expression of Cadherin-17 tended to be associated with intestinal type carcinoma, and carcinomas with Cadherin-17 expression was significantly more frequent in advanced stage cases than in early stage. Cadherin-17 is also a useful immunohistochemical marker for diagnosis of adenocarcinomas of the digestive system.