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Human DICER1 natural ORF mammalian expression plasmid

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Human DICER1 cDNA Clone Product Information
Gene_bank_ref_id:NM_030621.3
RefSeq ORF Size:5769bp
cDNA Description:Full length Clone DNA of Homo sapiens dicer 1, ribonuclease type I II.
Gene Synonym:DCR1, Dicer, HERNA, KIAA0928, DICER1
Species:Human
Vector:pCMV/hygro
Plasmid:pCMV-DICER1
Restriction Site:KpnI + XhoI (5.5kb + 5.77kb)
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 12T/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
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