|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MANF ORF mammalian expression plasmid, C-GFPSpark tag||HG11324-ACG|
|Human MANF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11324-ACR|
|Human MANF ORF mammalian expression plasmid, C-Flag tag||HG11324-CF|
|Human MANF ORF mammalian expression plasmid, C-His tag||HG11324-CH|
|Human MANF ORF mammalian expression plasmid, C-Myc tag||HG11324-CM|
|Human MANF ORF mammalian expression plasmid, C-HA tag||HG11324-CY|
|Human MANF Gene cDNA clone plasmid||HG11324-M|
|Human MANF ORF mammalian expression plasmid, N-Flag tag||HG11324-NF|
|Human MANF ORF mammalian expression plasmid, N-His tag||HG11324-NH|
|Human MANF ORF mammalian expression plasmid, N-Myc tag||HG11324-NM|
|Human MANF ORF mammalian expression plasmid, N-HA tag||HG11324-NY|
|Human MANF natural ORF mammalian expression plasmid||HG11324-UT|
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Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF and ARMET, is a secreted protein which belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types.