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Human HSPD1 Gene cDNA Clone (full-length ORF Clone)

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HSPD1cDNA Clone Product Information
Gene_bank_ref_id:NM_002156.4
cDNA Size:1722
cDNA Description:ORF Clone of Homo sapiens heat shock 60kDa protein 1 (chaperonin) DNA.
Gene Synonym:HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, HSPD1
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations 69 T/C and 273 A/G not causing the amino acid variation.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human HSPD1 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG11322-ACG$345
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Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG11322-CM$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG11322-CY$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG11322-M-F$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11322-M-N$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG11322-NF$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG11322-NH$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG11322-NM$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG11322-NY$315
Human HSPD1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11322-UT$315
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Background

HSPD1, also known as HSP60, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

References
  • Hansen J J, et al. (2002) Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet. 70: 1328-32.
  • Magen D, et al. (2008) Mitochondrial Hsp60 chaperonopathy causes an autosomal-recessive neurodegenerative disorder linked to brain hypomyelination and leukodystrophy. Am J Hum Genet. 83: 30-42.
  • Venner TJ, et al. (1990) Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 9 (8): 545-52.
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    Catalog:HG11322-M
    List Price: $115.00  (Save $0.00)
    Price:$115.00      [How to order]
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