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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human SOCS3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11315-ACG|
|Human SOCS3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11315-ACR|
|Human SOCS3 ORF mammalian expression plasmid, N-GFPSpark tag||HG11315-ANG|
|Human SOCS3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Flag-tagged||HG11315-CF|
|Human SOCS3 ORF mammalian expression plasmid, C-His tag||HG11315-CH|
|Human SOCS3 ORF mammalian expression plasmid, C-Myc tag||HG11315-CM|
|Human SOCS3 ORF mammalian expression plasmid, C-HA tag||HG11315-CY|
|Human SOCS3 ORF mammalian expression plasmid, N-Flag tag||HG11315-NF|
|Human SOCS3 ORF mammalian expression plasmid, N-His tag||HG11315-NH|
|Human SOCS3 ORF mammalian expression plasmid, N-Myc tag||HG11315-NM|
|Human SOCS3 ORF mammalian expression plasmid, N-HA tag||HG11315-NY|
|Human SOCS3 natural ORF mammalian expression plasmid||HG11315-UT|
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Suppressor of cytokine signaling 3, also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SOCS3 and CIS3, is a protein which is widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. SOCS3 / CIS3 contains one SH2 domain and one SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 / CIS3 is involved in negative regulation of cytokines that signal through the JAK / STAT pathway. SOCS3 / CIS3 inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. SOCS3 / CIS3 suppresses fetal liver erythropoiesis. It regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. SOCS3 / CIS3 regulates IL-6 signaling. SOCS3 / CIS3 interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. SOCS3 / CIS3 could be used as a possible therapeutic agent for treating rheumatoid arthritis.