|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ENPP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11308-ACG|
|Human ENPP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11308-ACR|
|Human ENPP2 ORF mammalian expression plasmid, C-Flag tag||HG11308-CF|
|Human ENPP2 ORF mammalian expression plasmid, C-His tag||HG11308-CH|
|Human ENPP2 ORF mammalian expression plasmid, C-Myc tag||HG11308-CM|
|Human ENPP2 ORF mammalian expression plasmid, C-HA tag||HG11308-CY|
|Human ENPP2 Gene cDNA clone plasmid||HG11308-M|
|Human ENPP2 ORF mammalian expression plasmid, N-Flag tag||HG11308-NF|
|Human ENPP2 ORF mammalian expression plasmid, N-His tag||HG11308-NH|
|Human ENPP2 ORF mammalian expression plasmid, N-Myc tag||HG11308-NM|
|Human ENPP2 ORF mammalian expression plasmid, N-HA tag||HG11308-NY|
|Human ENPP2 natural ORF mammalian expression plasmid||HG11308-UT|
|Learn more about expression Vectors|
ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.