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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CLU ORF mammalian expression plasmid, C-GFPSpark tag||HG11297-ACG|
|Human CLU ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11297-ACR|
|Human CLU ORF mammalian expression plasmid, C-Flag tag||HG11297-CF|
|Human CLU ORF mammalian expression plasmid, C-His tag||HG11297-CH|
|Human CLU ORF mammalian expression plasmid, C-Myc tag||HG11297-CM|
|Human CLU ORF mammalian expression plasmid, C-HA tag||HG11297-CY|
|Human CLU ORF mammalian expression plasmid, N-Flag tag||HG11297-NF|
|Human CLU ORF mammalian expression plasmid, N-His tag||HG11297-NH|
|Human CLU ORF mammalian expression plasmid, N-Myc tag||HG11297-NM|
|Human CLU ORF mammalian expression plasmid, N-HA tag||HG11297-NY|
|Human CLU natural ORF mammalian expression plasmid||HG11297-UT|
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Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.