|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ACOX1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11266-ACG|
|Human ACOX1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11266-ACR|
|Human ACOX1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11266-ANG|
|Human ACOX1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11266-ANR|
|Human ACOX1 ORF mammalian expression plasmid, C-Flag tag||HG11266-CF|
|Human ACOX1 ORF mammalian expression plasmid, C-His tag||HG11266-CH|
|Human ACOX1 ORF mammalian expression plasmid, C-Myc tag||HG11266-CM|
|Human ACOX1 ORF mammalian expression plasmid, C-HA tag||HG11266-CY|
|Human ACOX1 Gene cDNA clone plasmid||HG11266-M|
|Human ACOX1 ORF mammalian expression plasmid, N-Flag tag||HG11266-NF|
|Human ACOX1 ORF mammalian expression plasmid, N-His tag||HG11266-NH|
|Human ACOX1 ORF mammalian expression plasmid, N-Myc tag||HG11266-NM|
|Human ACOX1 ORF mammalian expression plasmid, N-HA tag||HG11266-NY|
|Human ACOX1 natural ORF mammalian expression plasmid||HG11266-UT|
|Learn more about expression Vectors|
Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.