|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AGO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11225-ACG|
|Human AGO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11225-ACR|
|Human AGO1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11225-ANG|
|Human AGO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11225-ANR|
|Human AGO1 ORF mammalian expression plasmid, C-Flag tag||HG11225-CF|
|Human AGO1 ORF mammalian expression plasmid, C-His tag||HG11225-CH|
|Human AGO1 ORF mammalian expression plasmid, C-Myc tag||HG11225-CM|
|Human AGO1 ORF mammalian expression plasmid, C-HA tag||HG11225-CY|
|Human AGO1 Gene cDNA clone plasmid||HG11225-M|
|Human AGO1 ORF mammalian expression plasmid, N-Flag tag||HG11225-NF|
|Human AGO1 ORF mammalian expression plasmid, N-His tag||HG11225-NH|
|Human AGO1 ORF mammalian expression plasmid, N-Myc tag||HG11225-NM|
|Human AGO1 ORF mammalian expression plasmid, N-HA tag||HG11225-NY|
|Human AGO1 natural ORF mammalian expression plasmid||HG11225-UT|
|Learn more about expression Vectors|
Protein argonaute-1, also known as eukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and ago subfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4. This genomic region is frequently lost in human cancers such as Wilms tumors, neuroblastoma, and carcinomas of the breast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed at low to medium levels. Differential polyadenylation and splicing result in a complex transcriptional pattern. EIF2C1 protein contains one PAZ domain and one Piwi domain. It is required for RNA-mediated gene silencing (RNAi) and transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them.