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Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone)

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APOH/B2G1cDNA Clone Product Information
Gene_bank_ref_id:NM_000042.2
cDNA Size:1038
cDNA Description:ORF Clone of Homo sapiens apolipoprotein H (beta-2-glycoprotein I) DNA.
Gene Synonym:BG, B2G1, APOH
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 796 G/T resulting in the amino acid 266Val substitution by Leu.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone) on other vectors
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG11221-ACG$325
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG11221-ACR$325
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG11221-CF$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG11221-CH$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG11221-CM$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG11221-CY$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG11221-M-F$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11221-M-N$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG11221-NF$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG11221-NH$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG11221-NM$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG11221-NY$295
Human Apolipoprotein H / APOH Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG11221-UT$295
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Background

Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

References
  • Kamboh MI, et al. (1998) Genetics of apolipoprotein H (beta2-glycoprotein I) and anionic phospholipid binding. Lupus. 7 Suppl 2: S10-3.
  • Singh P, et al. (2002) Genetics of apolipoprotein H (beta2-glycoprotein I) polymorphism in India. Ann Hum Biol. 29(3): 247-55.
  • Xia J, et al. (2004) Apolipoprotein H gene polymorphisms and risk of primary cerebral hemorrhage in a Chinese population. Cerebrovasc Dis. 17(2-3): 197-203.
  • Chen Q, et al. (2006) Complete DNA sequence variation in the apolipoprotein H (beta-glycoprotein I) gene and identification of informative SNPs. Ann Hum Genet. 70(Pt 1): 1-11.
  • Leduc MS, et al. (2008) Comprehensive evaluation of apolipoprotein H gene (APOH) variation identifies novel associations with measures of lipid metabolism in GENOA. J Lipid Res. 49(12): 2648-56.
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    Catalog:HG11221-M
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