|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PLA2G2A ORF mammalian expression plasmid, C-GFPSpark tag||HG11187-ACG|
|Human PLA2G2A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11187-ACR|
|Human PLA2G2A ORF mammalian expression plasmid, C-Flag tag||HG11187-CF|
|Human PLA2G2A ORF mammalian expression plasmid, C-His tag||HG11187-CH|
|Human PLA2G2A ORF mammalian expression plasmid, C-Myc tag||HG11187-CM|
|Human PLA2G2A ORF mammalian expression plasmid, C-HA tag||HG11187-CY|
|Human PLA2G2A Gene cDNA clone plasmid||HG11187-M|
|Human PLA2G2A ORF mammalian expression plasmid, N-Flag tag||HG11187-NF|
|Human PLA2G2A ORF mammalian expression plasmid, N-His tag||HG11187-NH|
|Human PLA2G2A ORF mammalian expression plasmid, N-Myc tag||HG11187-NM|
|Human PLA2G2A ORF mammalian expression plasmid, N-HA tag||HG11187-NY|
|Human PLA2G2A natural ORF mammalian expression plasmid||HG11187-UT|
|Learn more about expression Vectors|
Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein which belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.