|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ENTPD5 ORF mammalian expression plasmid, C-GFPSpark tag||HG11184-ACG|
|Human ENTPD5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11184-ACR|
|Human ENTPD5 ORF mammalian expression plasmid, C-Flag tag||HG11184-CF|
|Human ENTPD5 ORF mammalian expression plasmid, C-His tag||HG11184-CH|
|Human ENTPD5 ORF mammalian expression plasmid, C-Myc tag||HG11184-CM|
|Human ENTPD5 ORF mammalian expression plasmid, C-HA tag||HG11184-CY|
|Human ENTPD5 Gene cDNA clone plasmid||HG11184-M|
|Human ENTPD5 ORF mammalian expression plasmid, N-Flag tag||HG11184-NF|
|Human ENTPD5 ORF mammalian expression plasmid, N-His tag||HG11184-NH|
|Human ENTPD5 ORF mammalian expression plasmid, N-Myc tag||HG11184-NM|
|Human ENTPD5 ORF mammalian expression plasmid, N-HA tag||HG11184-NY|
|Human ENTPD5 natural ORF mammalian expression plasmid||HG11184-UT|
|Learn more about expression Vectors|
Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.