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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CADM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11168-ACG|
|Human CADM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11168-ACR|
|Human CADM1 ORF mammalian expression plasmid, C-Flag tag||HG11168-CF|
|Human CADM1 ORF mammalian expression plasmid, C-His tag||HG11168-CH|
|Human CADM1 ORF mammalian expression plasmid, C-Myc tag||HG11168-CM|
|Human CADM1 ORF mammalian expression plasmid, C-HA tag||HG11168-CY|
|Human CADM1 ORF mammalian expression plasmid, N-Flag tag||HG11168-NF|
|Human CADM1 ORF mammalian expression plasmid, N-His tag||HG11168-NH|
|Human CADM1 ORF mammalian expression plasmid, N-Myc tag||HG11168-NM|
|Human CADM1 ORF mammalian expression plasmid, N-HA tag||HG11168-NY|
|Human CADM1 natural ORF mammalian expression plasmid||HG11168-UT|
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Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression.