|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SH2D1A ORF mammalian expression plasmid, C-GFPSpark tag||HG11149-ACG|
|Human SH2D1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11149-ACR|
|Human SH2D1A ORF mammalian expression plasmid, N-GFPSpark tag||HG11149-ANG|
|Human SH2D1A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11149-ANR|
|Human SH2D1A ORF mammalian expression plasmid, C-Flag tag||HG11149-CF|
|Human SH2D1A ORF mammalian expression plasmid, C-His tag||HG11149-CH|
|Human SH2D1A ORF mammalian expression plasmid, C-Myc tag||HG11149-CM|
|Human SH2D1A ORF mammalian expression plasmid, C-HA tag||HG11149-CY|
|Human SH2D1A Gene cDNA clone plasmid||HG11149-M|
|Human SH2D1A ORF mammalian expression plasmid, N-Flag tag||HG11149-NF|
|Human SH2D1A ORF mammalian expression plasmid, N-His tag||HG11149-NH|
|Human SH2D1A ORF mammalian expression plasmid, N-Myc tag||HG11149-NM|
|Human SH2D1A ORF mammalian expression plasmid, N-HA tag||HG11149-NY|
|Human SH2D1A natural ORF mammalian expression plasmid||HG11149-UT|
|Learn more about expression Vectors|
SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome.