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Human MAP3K11 / MLK3 Gene cDNA Clone (full-length ORF Clone)

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MLK3cDNA Clone Product Information
Gene_bank_ref_id:NM_002419.3
cDNA Size:2544
cDNA Description:ORF Clone of Homo sapiens mitogen-activated protein kinase kinase kinase 11 DNA.
Gene Synonym:MLK3, PTK1, SPRK, MLK-3, MEKK11, MGC17114, MAP3K11
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for four point mutations: 141A/G not causing the amino acid variation, 281T/C resulting in the amino acid 94Ile substitution by thr, 944T/C resulting in the amino acid 315Leu substitution by Pro and 1919T/C resulting in the amino acid 640Leu substitution by Pro.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human MAP3K11 / MLK3 Gene cDNA Clone (full-length ORF Clone) on other vectors
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