|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CROT ORF mammalian expression plasmid, C-GFPSpark tag||HG11015-ACG|
|Human CROT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11015-ACR|
|Human CROT ORF mammalian expression plasmid, C-Flag tag||HG11015-CF|
|Human CROT ORF mammalian expression plasmid, C-His tag||HG11015-CH|
|Human CROT ORF mammalian expression plasmid, C-Myc tag||HG11015-CM|
|Human CROT ORF mammalian expression plasmid, C-HA tag||HG11015-CY|
|人 CROT 基因全长ORF克隆||HG11015-M|
|Human CROT natural ORF mammalian expression plasmid||HG11015-M-N|
|Human CROT ORF mammalian expression plasmid, N-Flag tag||HG11015-NF|
|Human CROT ORF mammalian expression plasmid, N-His tag||HG11015-NH|
|Human CROT ORF mammalian expression plasmid, N-Myc tag||HG11015-NM|
|Human CROT ORF mammalian expression plasmid, N-HA tag||HG11015-NY|
|Human CROT natural ORF mammalian expression plasmid||HG11015-UT|
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Carnitine octanoyltransferase (CROT or COT), also known as octanoyl-CoA: L-carnitine O-octanoyltransferase, medium-chain/long-chain carnitine acyltransferase, and carnitine medium-chain acyltransferase, is a carnitine acyltransferase belonging to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. Carnitine octanoyltransferase (CROT or COT) facilitate the transport of medium- and long-chain fatty acids through the peroxisomal and mitochondrial membranes. It is physiologically inhibited by malonyl-CoA. COT also has functions in efficiently converting one of the end products of the peroxisomal beta-oxidation of pristanic acid, 4, 8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.