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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human MS4A1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11007-ACG|
|Human MS4A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11007-ACR|
|Human MS4A1 ORF mammalian expression plasmid, C-Flag tag||HG11007-CF|
|Human MS4A1 ORF mammalian expression plasmid, C-His tag||HG11007-CH|
|Human MS4A1 ORF mammalian expression plasmid, C-Myc tag||HG11007-CM|
|Human MS4A1 ORF mammalian expression plasmid, C-HA tag||HG11007-CY|
|Human MS4A1 natural ORF mammalian expression plasmid||HG11007-M-N|
|Human MS4A1 ORF mammalian expression plasmid, N-Flag tag||HG11007-NF|
|Human MS4A1 ORF mammalian expression plasmid, N-His tag||HG11007-NH|
|Human MS4A1 ORF mammalian expression plasmid, N-Myc tag||HG11007-NM|
|Human MS4A1 ORF mammalian expression plasmid, N-HA tag||HG11007-NY|
|Human MS4A1 natural ORF mammalian expression plasmid||HG11007-UT|
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CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.