|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SERPINB8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10999-ACG|
|Human SERPINB8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10999-ACR|
|Human SERPINB8 ORF mammalian expression plasmid, N-GFPSpark tag||HG10999-ANG|
|Human SERPINB8 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10999-ANR|
|Human SERPINB8 ORF mammalian expression plasmid, C-Flag tag||HG10999-CF|
|Human SERPINB8 ORF mammalian expression plasmid, C-His tag||HG10999-CH|
|Human SERPINB8 ORF mammalian expression plasmid, C-Myc tag||HG10999-CM|
|Human SERPINB8 ORF mammalian expression plasmid, C-HA tag||HG10999-CY|
|Human SERPINB8 Gene cDNA clone plasmid||HG10999-M|
|Human SERPINB8 ORF mammalian expression plasmid, N-Flag tag||HG10999-NF|
|Human SERPINB8 ORF mammalian expression plasmid, N-His tag||HG10999-NH|
|Human SERPINB8 ORF mammalian expression plasmid, N-Myc tag||HG10999-NM|
|Human SERPINB8 ORF mammalian expression plasmid, N-HA tag||HG10999-NY|
|Human SERPINB8 natural ORF mammalian expression plasmid||HG10999-UT|
|Learn more about expression Vectors|
Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner.
Mouse SerpinB8, also known as Cytoplasmic antiproteinase 2, Peptidase inhibitor 8, SerpinB8, PI-8, SERPINB8 and CAP2, is a member of the Serpin superfamily. SERPINB8 was broadly expressed. In normal neuroendocrine tissues, strongest SerpinB8 expression was detected in islets of Langerhans of the pancreas. Moderate SerpinB8 expression was observed in neuroendocrine cells of the thyroid, adrenal cortex, colon, and pituitary gland. In the pancreas, SerpinB8 is specifically expressed by insulin-producing beta cells, and can be used as an additional diagnostic immunohistochemical marker. Mouse SerpinB8 distribution alters during kidney regeneration, possibly to control a prohormone convertase involved in inflammation or tissue repair.