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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CCL8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10989-ACG|
|Human CCL8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10989-ACR|
|Human CCL8 ORF mammalian expression plasmid, C-Flag tag||HG10989-CF|
|Human CCL8 ORF mammalian expression plasmid, C-His tag||HG10989-CH|
|Human CCL8 ORF mammalian expression plasmid, C-Myc tag||HG10989-CM|
|Human CCL8 ORF mammalian expression plasmid, C-HA tag||HG10989-CY|
|Human CCL8 ORF mammalian expression plasmid, Flag tag||HG10989-M-F|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-M-N|
|Human CCL8 ORF mammalian expression plasmid, N-Flag tag||HG10989-NF|
|Human CCL8 ORF mammalian expression plasmid, N-His tag||HG10989-NH|
|Human CCL8 ORF mammalian expression plasmid, N-Myc tag||HG10989-NM|
|Human CCL8 ORF mammalian expression plasmid, N-HA tag||HG10989-NY|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-UT|
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Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.