|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CCL8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10989-ACG|
|Human CCL8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10989-ACR|
|Human CCL8 ORF mammalian expression plasmid, C-Flag tag||HG10989-CF|
|Human CCL8 ORF mammalian expression plasmid, C-His tag||HG10989-CH|
|Human CCL8 ORF mammalian expression plasmid, C-Myc tag||HG10989-CM|
|Human CCL8 ORF mammalian expression plasmid, C-HA tag||HG10989-CY|
|Human CCL8 Gene cDNA clone plasmid||HG10989-M|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-M-N|
|Human CCL8 ORF mammalian expression plasmid, N-Flag tag||HG10989-NF|
|Human CCL8 ORF mammalian expression plasmid, N-His tag||HG10989-NH|
|Human CCL8 ORF mammalian expression plasmid, N-Myc tag||HG10989-NM|
|Human CCL8 ORF mammalian expression plasmid, N-HA tag||HG10989-NY|
|Human CCL8 natural ORF mammalian expression plasmid||HG10989-UT|
|Learn more about expression Vectors|
Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.