|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CLEC1B ORF mammalian expression plasmid, C-GFPSpark tag||HG10976-ACG|
|Human CLEC1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10976-ACR|
|Human CLEC1B ORF mammalian expression plasmid, C-Flag tag||HG10976-CF|
|Human CLEC1B ORF mammalian expression plasmid, C-His tag||HG10976-CH|
|Human CLEC1B ORF mammalian expression plasmid, C-Myc tag||HG10976-CM|
|Human CLEC1B ORF mammalian expression plasmid, C-HA tag||HG10976-CY|
|Human CLEC1B Gene cDNA clone plasmid||HG10976-M|
|Human CLEC1B ORF mammalian expression plasmid, N-Flag tag||HG10976-NF|
|Human CLEC1B ORF mammalian expression plasmid, N-His tag||HG10976-NH|
|Human CLEC1B ORF mammalian expression plasmid, N-Myc tag||HG10976-NM|
|Human CLEC1B ORF mammalian expression plasmid, N-HA tag||HG10976-NY|
|Human CLEC1B natural ORF mammalian expression plasmid||HG10976-UT|
|Learn more about expression Vectors|
CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.