pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human A2M ORF mammalian expression plasmid, C-GFPSpark tag||HG10952-ACG|
|Human A2M ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10952-ACR|
|Human A2M ORF mammalian expression plasmid, C-Flag tag||HG10952-CF|
|Human A2M ORF mammalian expression plasmid, C-His tag||HG10952-CH|
|Human A2M ORF mammalian expression plasmid, C-Myc tag||HG10952-CM|
|Human A2M ORF mammalian expression plasmid, C-HA tag||HG10952-CY|
|Human A2M natural ORF mammalian expression plasmid||HG10952-M-N|
|Human A2M ORF mammalian expression plasmid, N-Flag tag||HG10952-NF|
|Human A2M ORF mammalian expression plasmid, N-His tag||HG10952-NH|
|Human A2M ORF mammalian expression plasmid, N-Myc tag||HG10952-NM|
|Human A2M ORF mammalian expression plasmid, N-HA tag||HG10952-NY|
|Human A2M natural ORF mammalian expression plasmid||HG10952-UT|
|Learn more about expression Vectors|
alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is an abundant protein of the plasma of vertebrates and members of several invertebrate phyla and functions as a broad-spectrum protease-binding protein. alpha-2-macroglobulin is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. alpha-2-macroglobulin is a large plasma glycoprotein that has long been known as an irreversible inhibitor of a variety of proteinases. More recently, it has been reported that numerous growth factors, cytokines and hormones bind to alpha 2M through diverse mechanisms. A2M is also produced in the brain where it binds multiple extracellular ligands and is internalized by neurons and astrocytes. In the brain of Alzheimer's disease (AD) patients, A2M has been localized to diffuse amyloid plaques. A2M also binds soluble beta-amyloid, of which it mediates degradation. Protease-conjugated alpha2-macroglobulin is selectively bound by cells contacting the body fluids and alpha2-macroglobulin and its protease cargo are then internalized and degraded in secondary lysosomes of those cells. In addition to this function as an agent for protease clearance, alpha2-macroglobulin binds a variety of other ligands, including several peptide growth factors and modulates the activity of a lectin-dependent cytolytic pathway in arthropods.