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Human Nephronectin Gene cDNA Clone (full-length ORF Clone)

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NPNTcDNA Clone Product Information
Gene Bank Ref.ID:NM_001033047.1
cDNA Size:1698
cDNA Description:ORF Clone of Homo sapiens nephronectin DNA.
Gene Synonym:POEM, EGFL6L,
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 55 G/A resulting in the amino acid 19Ala substitution by Thr and 700 A/G resulting in the amino acid Ile substitution by Val.
Shipping Carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog:HG10932-M
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