|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ECE-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10887-ACG|
|Human ECE-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10887-ACR|
|Human ECE-1 ORF mammalian expression plasmid, C-Flag tag||HG10887-CF|
|Human ECE-1 ORF mammalian expression plasmid, C-His tag||HG10887-CH|
|Human ECE-1 ORF mammalian expression plasmid, C-Myc tag||HG10887-CM|
|Human ECE-1 ORF mammalian expression plasmid, C-HA tag||HG10887-CY|
|Human ECE-1 Gene cDNA clone plasmid||HG10887-M|
|Human ECE-1 ORF mammalian expression plasmid, N-Flag tag||HG10887-NF|
|Human ECE-1 ORF mammalian expression plasmid, N-His tag||HG10887-NH|
|Human ECE-1 ORF mammalian expression plasmid, N-Myc tag||HG10887-NM|
|Human ECE-1 ORF mammalian expression plasmid, N-HA tag||HG10887-NY|
|Human ECE-1 natural ORF mammalian expression plasmid||HG10887-UT|
|Learn more about expression Vectors|
Endothelin-converting enzyme 1, also known as ECE-1, is a single-pass type I I membrane protein which belongs to the peptidase M13 family. ECE-1 converts big endothelin-1 to endothelin-1. ECE-1 is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced from a single gene through the use of alternate promoters. These isoforms share the same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting.All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C of ECE-1 are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C of ECE-1 in liver, testis and small intestine; isoform B, C and D of ECE-1 in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction. It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.