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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human CD200 ORF mammalian expression plasmid, C-GFPSpark tag||HG10886-ACG|
|Human CD200 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10886-ACR|
|Human CD200 ORF mammalian expression plasmid, C-Flag tag||HG10886-CF|
|Human CD200 ORF mammalian expression plasmid, C-His tag||HG10886-CH|
|Human CD200 ORF mammalian expression plasmid, C-Myc tag||HG10886-CM|
|Human CD200 ORF mammalian expression plasmid, C-HA tag||HG10886-CY|
|Human CD200 ORF mammalian expression plasmid, N-Flag tag||HG10886-NF|
|Human CD200 ORF mammalian expression plasmid, N-His tag||HG10886-NH|
|Human CD200 ORF mammalian expression plasmid, N-Myc tag||HG10886-NM|
|Human CD200 ORF mammalian expression plasmid, N-HA tag||HG10886-NY|
|Human CD200 natural ORF mammalian expression plasmid||HG10886-UT|
|Learn more about expression Vectors|
CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in a number of human malignancies. In addition, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.