|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CHRDL1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10866-ACG|
|Human CHRDL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10866-ACR|
|Human CHRDL1 ORF mammalian expression plasmid, C-Flag tag||HG10866-CF|
|Human CHRDL1 ORF mammalian expression plasmid, C-His tag||HG10866-CH|
|Human CHRDL1 ORF mammalian expression plasmid, C-Myc tag||HG10866-CM|
|Human CHRDL1 ORF mammalian expression plasmid, C-HA tag||HG10866-CY|
|Human CHRDL1 Gene cDNA clone plasmid||HG10866-M|
|Human CHRDL1 ORF mammalian expression plasmid, N-Flag tag||HG10866-NF|
|Human CHRDL1 ORF mammalian expression plasmid, N-His tag||HG10866-NH|
|Human CHRDL1 ORF mammalian expression plasmid, N-Myc tag||HG10866-NM|
|Human CHRDL1 ORF mammalian expression plasmid, N-HA tag||HG10866-NY|
|Human CHRDL1 natural ORF mammalian expression plasmid||HG10866-UT|
|Learn more about expression Vectors|
Cordin-like 1 (CHRDL1) is an antagonist of bone morphogenetic protein 4. It may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Cordin-like 1 (CHRDL1) is an inhibitor of the bone morphogenetic proteins (BMPs) that have functions in inducing ectopic bone formation. CHRDL1 acheves its inhibiting ability through affecting the osteogenic differentiation of MC3T3-E1 cells. Besides its function as an antagonist, CHRDL1 enhanced the proliferation of human mesenchymal stem cells in a BMP2-independent manner.