|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PLA2G1B ORF mammalian expression plasmid, C-GFPSpark tag||HG10865-ACG|
|Human PLA2G1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10865-ACR|
|Human PLA2G1B ORF mammalian expression plasmid, C-Flag tag||HG10865-CF|
|Human PLA2G1B ORF mammalian expression plasmid, C-His tag||HG10865-CH|
|Human PLA2G1B ORF mammalian expression plasmid, C-Myc tag||HG10865-CM|
|Human PLA2G1B ORF mammalian expression plasmid, C-HA tag||HG10865-CY|
|Human PLA2G1B Gene cDNA clone plasmid||HG10865-M|
|Human PLA2G1B ORF mammalian expression plasmid, N-Flag tag||HG10865-NF|
|Human PLA2G1B ORF mammalian expression plasmid, N-His tag||HG10865-NH|
|Human PLA2G1B ORF mammalian expression plasmid, N-Myc tag||HG10865-NM|
|Human PLA2G1B ORF mammalian expression plasmid, N-HA tag||HG10865-NY|
|Human PLA2G1B natural ORF mammalian expression plasmid||HG10865-UT|
|Learn more about expression Vectors|
Mouse phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.