|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HDAC8 ORF mammalian expression plasmid, C-GFPSpark tag||HG10864-ACG|
|Human HDAC8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10864-ACR|
|Human HDAC8 ORF mammalian expression plasmid, N-GFPSpark tag||HG10864-ANG|
|Human HDAC8 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10864-ANR|
|Human HDAC8 ORF mammalian expression plasmid, C-Flag tag||HG10864-CF|
|Human HDAC8 ORF mammalian expression plasmid, C-His tag||HG10864-CH|
|Human HDAC8 ORF mammalian expression plasmid, C-Myc tag||HG10864-CM|
|Human HDAC8 ORF mammalian expression plasmid, C-HA tag||HG10864-CY|
|Human HDAC8 Gene cDNA clone plasmid||HG10864-M|
|Human HDAC8 ORF mammalian expression plasmid, N-Flag tag||HG10864-NF|
|Human HDAC8 ORF mammalian expression plasmid, N-His tag||HG10864-NH|
|Human HDAC8 ORF mammalian expression plasmid, N-Myc tag||HG10864-NM|
|Human HDAC8 ORF mammalian expression plasmid, N-HA tag||HG10864-NY|
|Human HDAC8 natural ORF mammalian expression plasmid||HG10864-UT|
|Learn more about expression Vectors|
Histone deacetylase 8, also known as HDAC8 and HDACL1, is a nucleus and cytoplasm protein which belongs to the histone deacetylase family and HD type 1 subfamily. Histone deacetylases (HDACs) are a growing family of enzymes implicated in transcriptional regulation by affecting the acetylation state of core histones in the nucleus of cells. HDAC8 / HDACL1 is weakly expressed in most tissues. It expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. HDAC8 / HDACL1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones ( H2A, H2B, H3 and H4 ). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 / HDACL1 may play a role in smooth muscle cell contractility. HDAC8 / HDACL1 may be a potential drug target for neuroblastoma differentiation therapy using selective inhibitors, avoiding unspecific side effects.