|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human YWHAB ORF mammalian expression plasmid, C-GFPSpark tag||HG10843-ACG|
|Human YWHAB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10843-ACR|
|Human YWHAB ORF mammalian expression plasmid, N-GFPSpark tag||HG10843-ANG|
|Human YWHAB ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10843-ANR|
|Human YWHAB ORF mammalian expression plasmid, C-Flag tag||HG10843-CF|
|Human YWHAB ORF mammalian expression plasmid, C-His tag||HG10843-CH|
|Human YWHAB ORF mammalian expression plasmid, C-Myc tag||HG10843-CM|
|Human YWHAB ORF mammalian expression plasmid, C-HA tag||HG10843-CY|
|Human YWHAB Gene cDNA clone plasmid||HG10843-M|
|Human YWHAB ORF mammalian expression plasmid, N-Flag tag||HG10843-NF|
|Human YWHAB ORF mammalian expression plasmid, N-His tag||HG10843-NH|
|Human YWHAB ORF mammalian expression plasmid, N-Myc tag||HG10843-NM|
|Human YWHAB ORF mammalian expression plasmid, N-HA tag||HG10843-NY|
|Human YWHAB natural ORF mammalian expression plasmid||HG10843-UT|
|Learn more about expression Vectors|
14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.