|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human P4HB / DSI ORF mammalian expression plasmid, C-GFPSpark tag||HG10827-ACG|
|Human P4HB / DSI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10827-ACR|
|Human P4HB / DSI ORF mammalian expression plasmid, C-Flag tag||HG10827-CF|
|Human P4HB / DSI ORF mammalian expression plasmid, C-His tag||HG10827-CH|
|Human P4HB / DSI ORF mammalian expression plasmid, C-Myc tag||HG10827-CM|
|Human P4HB / DSI ORF mammalian expression plasmid, C-HA tag||HG10827-CY|
|Human P4HB / DSI Gene cDNA clone plasmid||HG10827-M|
|Human P4HB / DSI ORF mammalian expression plasmid, N-Flag tag||HG10827-NF|
|Human P4HB / DSI ORF mammalian expression plasmid, N-His tag||HG10827-NH|
|Human P4HB / DSI ORF mammalian expression plasmid, N-Myc tag||HG10827-NM|
|Human P4HB / DSI ORF mammalian expression plasmid, N-HA tag||HG10827-NY|
|Human P4HB / DSI natural ORF mammalian expression plasmid||HG10827-UT|
|Learn more about expression Vectors|
Mouse protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.