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Human MAP3K8 Gene cDNA Clone (full-length ORF Clone)

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MAP3K8cDNA Clone Product Information
Gene_bank_ref_id:NM_005204.2
cDNA Size:1404
cDNA Description:ORF Clone of Homo sapiens mitogen-activated protein kinase kinase kinase 8 DNA.
Gene Synonym:COT, EST, ESTF, TPL2, Tpl-2, c-COT, FLJ10486
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10800-ACG$325
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10800-ACR$325
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10800-ANG$325
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10800-ANR$325
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10800-CF$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10800-CH$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10800-CM$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10800-CY$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG10800-M-F$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, His-taggedHG10800-M-H$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10800-M-N$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10800-NF$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10800-NH$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10800-NM$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10800-NY$295
Human MAP3K8 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10800-UT$295
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Background

Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).

References
  • Clark,A.M. et al., 2004, Genes Chromosomes Cancer. 41 (2):99-108.
  • Chan,H. et al., 2005, Biochem Biophys Res Commun. 328 (1):198-205.
  • Aparecida Alves,C. et al., 2006, Eur J Gynaecol Oncol. 27 (6):589-93.
  • Mielke,L.A. et al., 2009, J Immunol. 183 (12):7984-93.
  • Glossop,J.R. et al., 2009,Gene Expr Patterns  9 (5):381-8. 
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    Catalog:HG10800-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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