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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human GP1BB ORF mammalian expression plasmid, C-GFPSpark tag||HG10742-ACG|
|Human GP1BB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10742-ACR|
|Human GP1BB ORF mammalian expression plasmid, C-Flag tag||HG10742-CF|
|Human GP1BB ORF mammalian expression plasmid, C-His tag||HG10742-CH|
|Human GP1BB ORF mammalian expression plasmid, C-Myc tag||HG10742-CM|
|Human GP1BB ORF mammalian expression plasmid, C-HA tag||HG10742-CY|
|Human GP1BB ORF mammalian expression plasmid, Flag tag||HG10742-M-F|
|Human GP1BB ORF mammalian expression plasmid, N-Flag tag||HG10742-NF|
|Human GP1BB ORF mammalian expression plasmid, N-His tag||HG10742-NH|
|Human GP1BB ORF mammalian expression plasmid, N-Myc tag||HG10742-NM|
|Human GP1BB ORF mammalian expression plasmid, N-HA tag||HG10742-NY|
|Human GP1BB natural ORF mammalian expression plasmid||HG10742-UT|
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Platelet glycoprotein Ib (GPIb) complex is best known as a major platelet receptor for von Willebrand factor essential for platelet adhesion under high shear conditions found in arteries and in thrombosis. The GPIb complex is composed of GPIb alpha (Platelet glycoprotein Ib alpha chain) covalently attached to GPIb beta (Platelet glycoprotein Ib beta chain) and noncovalently complexed with GPIX and GPV. GPIb-beta, also known as GP1BB, CD42b-beta and CD42c, is single-pass type I membrane protein expressed in heart and brain, which is a critical component of the von Willebrand factor (vWF) receptor. The cysteine knot region of GPIb beta in the N terminus is critical for the conformation of GPIb beta that interacts with GPIX. The precursor of GP1BB is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. GPIb is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. GPIb alpha chain provides the vWF binding site, and GPIb beta chain contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. GP1BB is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (vWF), and mediates platelet adhesion in the arterial circulation. Defects in GP1BB are a cause of Bernard-Soulier syndrome (BSS), also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.