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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human ULBP-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10679-ACG|
|Human ULBP-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10679-ACR|
|Human ULBP-1 ORF mammalian expression plasmid, C-Flag tag||HG10679-CF|
|Human ULBP-1 ORF mammalian expression plasmid, C-His tag||HG10679-CH|
|Human ULBP-1 ORF mammalian expression plasmid, C-Myc tag||HG10679-CM|
|Human ULBP-1 ORF mammalian expression plasmid, C-HA tag||HG10679-CY|
|Human ULBP-1 ORF mammalian expression plasmid, Flag tag||HG10679-M-F|
|Human ULBP-1 ORF mammalian expression plasmid, N-Flag tag||HG10679-NF|
|Human ULBP-1 ORF mammalian expression plasmid, N-His tag||HG10679-NH|
|Human ULBP-1 ORF mammalian expression plasmid, N-Myc tag||HG10679-NM|
|Human ULBP-1 ORF mammalian expression plasmid, N-HA tag||HG10679-NY|
|Human ULBP-1 natural ORF mammalian expression plasmid||HG10679-UT|
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UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins, and have tissue-specific expressions. ULBP1, also known as RAET1I and NKG2DL1, together with at least ULBP 2 and 3, are well-known ligands for NKG2D, and activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver and bone marrow, as well as some tumor cells. As an unconventional member of the MHC class I family, ULBP1 function in immune responses, especially in cancer and infectious diseases. Unlike other ULBP members, ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor, and thus might escape the immune surveillance. Furthermore, UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.
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