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Human CDC37 Gene cDNA Clone (full-length ORF Clone)

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CDC37cDNA Clone Product Information
Gene_bank_ref_id:NM_007065.3
cDNA Size:1137
cDNA Description:ORF Clone of Homo sapiens cell division cycle 37 homolog (S. cerevisiae) DNA.
Gene Synonym:P50CDC37
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human CDC37 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10647-ACG$325
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10647-ACR$325
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10647-ANG$325
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10647-ANR$325
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10647-CF$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10647-CH$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10647-CM$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10647-CY$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-taggedHG10647-M-F$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10647-M-N$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10647-NF$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10647-NH$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10647-NM$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10647-NY$295
Human CDC37 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10647-UT$295
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Background

CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

References
  • Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4.
  • Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61.
  • Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.
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    Catalog:HG10647-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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