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Human PKN2 Gene cDNA Clone (full-length ORF Clone)

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Human PKN2 cDNA Clone Product Information
NCBI RefSeq:NM_006256.2
RefSeq ORF Size:2955
cDNA Description:ORF Clone of Homo sapiens protein kinase N2 DNA.
Gene Synonym:PAK2, PRK2, Pak-2, PRKCL2, PRO2042, MGC71074, MGC150606
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1677 T/C and 421T/C not causing the amino acid variation.
Antibiotic in E.coli:Ampicilin
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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