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Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone)

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BIRC4/XIAPcDNA Clone Product Information
Gene_bank_ref_id:NM_001167.2
cDNA Size:1494
cDNA Description:ORF Clone of Homo sapiens X-linked inhibitor of apoptosis DNA.
Gene Synonym:API3, ILP1, MIHA, XLP2, BIRC4
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 1268A/C resulting in the amino acid 423Gln substitution by Pro.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10606-ACG$325
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10606-ACR$325
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10606-ANG$325
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10606-ANR$325
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10606-CF$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10606-CH$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10606-CM$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10606-CY$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10606-M-N$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10606-NF$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10606-NH$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10606-NM$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10606-NY$295
Human XIAP / BIRC4 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10606-UT$295
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Background

E3 ubiquitin-protein ligase XIAP / BIRC4, also known as inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP / BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP / BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP / BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP / BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2). XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

References
  • Holcik M, et al. (2000) Functional Characterization of the X-Linked Inhibitor of Apoptosis (XIAP) Internal Ribosome Entry Site Element: Role of La Autoantigen in XIAP Translation. Mol Cell Biol. 20 (13): 4648-57.
  • Winsauer G, et al. (2008) XIAP regulates bi-phasic NF-kappaB induction involving physical interaction and ubiquitination of MEKK2. Cell Signal. 20 (11): 2107-12.
  • Suzuki Y, et al. (2001) X-linked inhibitor of apoptosis protein (XIAP) inhibits caspase-3 and -7 in distinct modes. J Biol Chem. 276 (29): 27058-63.
  • Size / Price
    Catalog:HG10606-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
    Availability5 business days
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