|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AREG ORF mammalian expression plasmid, C-GFPSpark tag||HG10558-ACG|
|Human AREG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10558-ACR|
|Human AREG ORF mammalian expression plasmid, C-Flag tag||HG10558-CF|
|Human AREG ORF mammalian expression plasmid, C-His tag||HG10558-CH|
|Human AREG ORF mammalian expression plasmid, C-Myc tag||HG10558-CM|
|Human AREG ORF mammalian expression plasmid, C-HA tag||HG10558-CY|
|Human AREG Gene cDNA clone plasmid||HG10558-M|
|Human AREG ORF mammalian expression plasmid, N-Flag tag||HG10558-NF|
|Human AREG ORF mammalian expression plasmid, N-His tag||HG10558-NH|
|Human AREG ORF mammalian expression plasmid, N-Myc tag||HG10558-NM|
|Human AREG ORF mammalian expression plasmid, N-HA tag||HG10558-NY|
|Human AREG natural ORF mammalian expression plasmid||HG10558-UT|
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Amphiregulin(AREG), also known as colorectum cell-derived growth factor, CRDGF, SDGF and AREG, is a member of the epidermal growth factor family and the amphiregulin family. Members of EGF family are cytokines that include 10 proteins such as EGF, TGFb, HBEGF, and the various heregulins. Amphiregulin is a single-pass membrane protein and contains one EGF-like domain. It plays a key role in epithelial development in various organs. Amphiregulin has a realationship with epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. As a bifunctional growth-modulating glycoprotein, amphiregulin inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. Amphiregulin also may play a protective role in bleomycin-induced pneumopathy, probably through the activation of survival signals.