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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human MMP-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10532-ACG|
|Human MMP-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10532-ACR|
|Human MMP-1 ORF mammalian expression plasmid, C-Flag tag||HG10532-CF|
|Human MMP-1 ORF mammalian expression plasmid, C-His tag||HG10532-CH|
|Human MMP-1 ORF mammalian expression plasmid, C-Myc tag||HG10532-CM|
|Human MMP-1 ORF mammalian expression plasmid, C-HA tag||HG10532-CY|
|Human MMP-1 ORF mammalian expression plasmid, N-Flag tag||HG10532-NF|
|Human MMP-1 ORF mammalian expression plasmid, N-His tag||HG10532-NH|
|Human MMP-1 ORF mammalian expression plasmid, N-Myc tag||HG10532-NM|
|Human MMP-1 ORF mammalian expression plasmid, N-HA tag||HG10532-NY|
|Human MMP-1 natural ORF mammalian expression plasmid||HG10532-UT|
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MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.