Quick Order

Human CystatinB Gene cDNA Clone (full-length ORF Clone)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Cystatin B/CSTBcDNA Clone Product Information
Gene_bank_ref_id:NM_000100.2
cDNA Size:297
cDNA Description:ORF Clone of Homo sapiens cystatin B (stefin B) DNA.
Gene Synonym:PME, CST6, EPM1, STFB, CSTB
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human CystatinB Gene cDNA Clone (full-length ORF Clone) on other vectors
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10435-ACG$325
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10435-ACR$325
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10435-ANG$325
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10435-ANR$325
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10435-CF$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10435-CH$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10435-CM$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10435-CY$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10435-M-F$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10435-M-N$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10435-NF$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10435-NH$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10435-NM$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10435-NY$295
Human CystatinB Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10435-UT$295
 Learn more about expression Vectors
Related Products
Product nameProduct name
Background

Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Size / Price
Catalog:HG10435-M
List Price: $95.00  (Save $0.00)
Price:$95.00      [How to order]
Availability5 business days
Images
    Recently Viewed Items
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"