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Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone)

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HFE2cDNA Clone Product Information
Gene_bank_ref_id:NM_213653.3
cDNA Size:1281
cDNA Description:ORF Clone of Homo sapiens hemochromatosis type 2 (juvenile) (HFE2), transcript variant a DNA.
Gene Synonym:JH, HJV, RGMC, HFE2A, MGC23953, HFE2
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone) on other vectors
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10410-ACG$325
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10410-ACR$325
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10410-CF$295
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Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10410-CY$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10410-M-F$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10410-M-N$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10410-NF$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10410-NH$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10410-NM$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10410-NY$295
Human HFE2 / RGM-C transcript variant a Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10410-UT$295
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Background

Hemojuvelin, also known as HFE2, is a membrane-bound and soluble protein which belongs to the repulsive guidance molecule (RGM) family. It is known that RGMs function through Neogenin, a homologue of the Netrin receptor deleted in colon cancer. In mammals, RGM family consists of three glycoproteins which have discrete expression patterns and functions (RGM-A, RGM-B, and RGM-C). Hemojuvelin is expressed in adult and fetal liver, heart, and skeletal muscle. Hemojuvelin acts as a bone morphogenetic protein (BMP) coreceptor. Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism. It plays a key role in iron metabolism. Hemojuvelin represents the cellular receptor for hepcidin. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. Defects in hemojuvelin are the cause of hemochromatosis type 2A, also known as juvenile hemochromatosis (JH).

References
  • Papanikolaou G, et al. (2004) Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis. Nat Genet. 36(1):77-82.
  • Babitt JL, et al. (2006) Bone morphogenetic protein signaling by hemojuvelin regulates hepcidin expression. Nat Genet. 38(5):531-9.
  • Zhang AS, et al. (2008) Neogenin-mediated hemojuvelin shedding occurs after hemojuvelin traffics to the plasma membrane. J Biol Chem. 283(25):17494-502.
  • Size / Price
    Catalog:HG10410-M
    List Price: $95.00  (Save $0.00)
    Price:$95.00      [How to order]
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