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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human SLITRK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10340-ACG|
|Human SLITRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10340-ACR|
|Human SLITRK1 ORF mammalian expression plasmid, C-Flag tag||HG10340-CF|
|Human SLITRK1 ORF mammalian expression plasmid, C-His tag||HG10340-CH|
|Human SLITRK1 ORF mammalian expression plasmid, C-Myc tag||HG10340-CM|
|Human SLITRK1 ORF mammalian expression plasmid, C-HA tag||HG10340-CY|
|Human SLITRK1 natural ORF mammalian expression plasmid||HG10340-M-N|
|Human SLITRK1 ORF mammalian expression plasmid, N-Flag tag||HG10340-NF|
|Human SLITRK1 ORF mammalian expression plasmid, N-His tag||HG10340-NH|
|Human SLITRK1 ORF mammalian expression plasmid, N-Myc tag||HG10340-NM|
|Human SLITRK1 ORF mammalian expression plasmid, N-HA tag||HG10340-NY|
|Human SLITRK1 natural ORF mammalian expression plasmid||HG10340-UT|
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SLITRK1 (Slit and Trk-like family member 1) is a integral membrane protein belonging to the SLITRK family consists of at least 6 members (SLITRK1-6). They are named and characterized by the presence of two leucine-rich repeats (LRRs) in the extracellular domain similar to those found in a secreted axonal growth-controlling protein, Slit, as well as a C-terminal domain with homology to Trk neurotrophin tyrosine kinase receptors. Expression of SLITRKs are highly restricted to neural tissues, and are identified as the neuronal components modulating the neurite outgrowth. More specifically, SLITRK1 expression is found in the mature neurons of the cerebrum, thalamus and hippocampus, and induces unipolar neurites in cultured neuronal cells. Human SLITRK1 is a 696 amino acid precursor protein, and one truncating frameshift mutation (448 aa) has been linked to Tourette's syndrome, a genetically influenced developmental neuropsychiatric disorder characterized by chronic vocal and motor tics. In addition, all SLITRK genes are differentially expressed in brain tumors, such as astrocytoma, oligodendroglioma, glioblastoma, and are suggested to be useful molecular indicators of brain tumor properties.
1. Aruga, J. and Mikoshiba, K. 2003, Mol. Cell. Neurosci. 24: 117-129.
2. Aruga, J. et al., 2003, Gene. 315: 87-94.
3. Abelson, J.F. et al., 2005, Science. 310: 317-320.
4. Grados, M.A. and Walkup. J.T. 2006, Trends. Genet. 22: 291-293.