|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-GFPSpark tag||HG10335-ACG|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10335-ACR|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-Flag tag||HG10335-CF|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-His tag||HG10335-CH|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-Myc tag||HG10335-CM|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, C-HA tag||HG10335-CY|
|Human CD62E / E-Selectin Gene cDNA clone plasmid||HG10335-M|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, N-Flag tag||HG10335-NF|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, N-His tag||HG10335-NH|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, N-Myc tag||HG10335-NM|
|Human CD62E / E-Selectin ORF mammalian expression plasmid, N-HA tag||HG10335-NY|
|Human CD62E / E-Selectin natural ORF mammalian expression plasmid||HG10335-UT|
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E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.