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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human HGFA ORF mammalian expression plasmid, C-GFPSpark tag||HG10329-ACG|
|Human HGFA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10329-ACR|
|Human HGFA ORF mammalian expression plasmid, C-Flag tag||HG10329-CF|
|Human HGFA ORF mammalian expression plasmid, C-His tag||HG10329-CH|
|Human HGFA ORF mammalian expression plasmid, C-Myc tag||HG10329-CM|
|Human HGFA ORF mammalian expression plasmid, C-HA tag||HG10329-CY|
|Human HGFA ORF mammalian expression plasmid, N-Flag tag||HG10329-NF|
|Human HGFA ORF mammalian expression plasmid, N-His tag||HG10329-NH|
|Human HGFA ORF mammalian expression plasmid, N-Myc tag||HG10329-NM|
|Human HGFA ORF mammalian expression plasmid, N-HA tag||HG10329-NY|
|Human HGFA natural ORF mammalian expression plasmid||HG10329-UT|
|Learn more about expression Vectors|
HGF activator (HGFA) is a serum-derived serine protease and belongs to the peptidase family S1.HGFA is responsible for the conversion of hepatocyte growth factor (HGF), from the inactive single-chain precursor to the active heterodimeric form, which is a potent mitogen, motogen, and morphogen for liver cells, epithelial cells, and endothelial cells. HGFA is synthesized and secreted by the liver and circulates in the plasma as an inactive single-chain zymogen in normal states. The zymogen is cleaved by thrombin or thermolysin through the endoproteolytic process and forms an active heterodimer linked by a disulfide bond. In turn, the active protease can be inhibited by HGFA inhibitors (HAIs) including HAI-1 and HAI-2. In addition, the HGFA zymogen acquires a strong affinity upon activation and thus may ensure the local action in tissue regeneration in liver, kidney and skin. It has been reported that activation of HGF is a critical limiting step in the HGF/SF-induced signaling pathway mediated by Met, and accordingly, aberrant expression of HGFA is implicated in tumorigenesis and progression.
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